Figure 2.

Effects of CBD on mitochondrial structure in animal cells. B104 are a mouse neuroblastomacell line. Mitochondria were visualised using Mitotracker© green after 3 hours. The images are typical cells taken at high magnification using an oil lens. At 3 µM CBD induces formation of small loop structures, with some evidence of fusion and the formation of donuts, characteristic of an initial stress response. At 9 µM it induces fission, with evidence of some mild mitochondrial swelling. At 27 µM it seems to be inducing massive mitochondrial swelling. This can be partly explained by modulation of calcium flux, and in part, could be related to the effects of CBD on VDAC1. This is data taken from the authors published abstract (referenced in main text)