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. 2020 Apr 30;59(31):12837–12841. doi: 10.1002/anie.202000148

Figure 1.

Figure 1

Effects of LL‐37 on IAPP amyloid self‐assembly and cell‐damaging effects. a) Fibrillogenesis of IAPP (16.5 μm) alone or with LL‐37 (1:1) determined by ThT binding (means±SD, 3 assays). LL‐37 alone is shown for comparison (1 assay). b) TEM images of 7 days aged solutions from (a) as indicated (bars, 100 nm); inset in LL‐37 image shows LL‐37 fibrils (minor population). c) Cell viability of cultured RIN5fm cells after treatment with IAPP and its mixtures from 1a (7 days aged) determined by MTT reduction [mean±SD, 3 assays (n=3 each)]; effects of LL‐37 alone are also shown (1 assay, n=3). d) IC50 of inhibitory effect of LL‐37 on IAPP cytotoxicity determined by titration of IAPP (100 nm; red symbol) with LL‐37 and MTT reduction [mean±SD, 3 titration assays (n=3 each)]. e) Fibrillogenesis of IAPP (16.5 μm) alone or with LL‐37 (1:1) following seeding with fIAPP (10 %) determined by ThT binding (mean±SD, 3 assays).