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Expression patterns of differentially expressed genes in different symbiont‐infected lines. (A) Doubly infected (Iws) compared with uninfected (U) lines. (B) Iws compared with singly Spiroplasma‐infected (Is) lines. (C) Iws compared with singly Wolbachia‐infected (Iw) lines. Relative expression was calculated using the 2−ΔΔCt method. Data are presented as mean ± SEM.
