Figure 2.

The passenger mRFP carrying a ST interacts with Lpp‐OmpA‐eGFP‐SC and is translocated to the E. coli cell envelope. E. coli cells harboring plasmids pET_mRFP‐ST and pTF16_Lpp‐OmpA‐eGFP‐SC were either A) induced with IPTG and l‐arabinose for translocation of mRFP‐ST to the cell surface after covalent interaction with Lpp‐OmpA‐eGFP‐SC or B) induced with only IPTG for cytosolic expression of mRFP‐ST. Fluorescence microscopy pictures were taken 6 h after induction. Scale bars of magnified insets: 1 μm.