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Development and characterization of hepcidin C-ELISA. (A) Dose response curve starting at 400 ng/mL with serial dilutions. Structurally similar peptides were compared to assess selectivity. Hepcidin-10 to -25 is an N-terminally truncated hepcidin peptide. (B) Comparison of monoclonal hepcidin C-ELISA (n = 292) with a previously described polyclonal C-ELISA (1).
