Fig. 3.

a Laser-scanning immunofluorescence micrographs of CaCo2 WT and AP1S1 KO cells. Tight-junction protein ZO-1 shows aberrant patchy localization upon AP1S1 deletion and in cells expressing patient mutations (AP1S1-T269C and AP1S1-G346A) but not AP1S1-WT (b). Basolateral E-cadherin staining is not affected by AP1S1. Scale bar 10 µm, arrowheads indicate respective plane in XY and XZ