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. 2020 Apr 30;66(5):957–969. doi: 10.1007/s00294-020-01080-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Karyotypes of parental strains, hybrids and spore clones. a Karyotypes of a two-species kudvarum hybrid, a three-species cekudvarum hybrid and the parental strains. Only the regions of smaller chromosomes are shown in which individual bands can be distinguished in the cekudvarum karyotype. b Karyotypes of two-species cevarum hybrids and spore clones. Only the regions containing the bands of the MAT-carrying chromosomes are shown. The columns on the left sides of the gel photographs show the conventional numbering of chromosomes in the S. cerevisiae, S. uvarum and S. kudriavzevii genomes. The S. uvarum chromosomes are numbered according to Nguyen et al (2000). Sc: S. cerevisiae 10-170; Sk: S. kudriavzevii 10-1653; Su: S. uvarum 10-522; II/6: kudvarum hybrid; II/6.1: cekudvarum hybrid; A27 and A3: cevarum hybrids, A3/1a: sterile spore clone of A3; A3/1b: fertile spore clone of A3. Arrowhead marks the position of the missing Chr III^Su (Chr 2) in the fertile spore clone A3/1b