Table 2.
Primers used for the identification of the MAT cassettes of the species
| Species | Primer | Sequence | Amplicon size (bp) | |
|---|---|---|---|---|
| S. cerevisiae | MATaSc | F: CCACATTAAAAAAGAGAAGAGC | MATaSc–Scout: | 509 |
| MATalphaSc | F: TAAAATCCAAATTCACAGGATAGCGTCT | MATalphaSc- Scout: | 672 | |
| Scout | R: TATGGTTAAGATAAGAACAAAGAATG | |||
| S. kudriavzevii | MATaSk | F: GTATGAAAAATCAAGCTAA | MATaSk- Skout: | 309 |
| MATalphaSk | F: GTAATGGCATAGTGAAACGAATAAGT | MATalphaSk- Skout: | 657 | |
| Skout | R: GTAAATACCTCAAAGGAATTATCA | |||
| S. uvarum | MATaSu | F: CAACGTGAATCAATCCTAA | MATaSu- Suout: | 439 |
| MATalphaSu | F: TCGAGAAAAGCATCAATAACAC | MATalphaSu- Suout: | 603 | |
| Suout | R: TCACCAAATACGAAAAGTAA | |||