Fig. 10. Phosphorus (green, (A)) and sulfur (yellow, (B)–(E)) -detected chromatograms of cytosol FTSs. (A) From WT cells grown with 40 μM Fe and 10 μM Cu; isolated using buffer (C). (B) 10 mM MES buffer. (C) Same as in (A). (D) WT cells grown as in (A) but cytosol was isolated using 20 mM ammonium acetate. (E) Reduced glutathione (250 μM). A single treated column was used.
