Fig. 2. Iron-detected chromatograms of FTS from dilute isolated cytosol. Top panel: The average of 2, 4, 4, 8, and 1 independent traces are shown, respectively. The top three samples were prepared from WT cells grown with 10 000, 100, 40, and 1 μM FeIII citrate added to the growth medium. Trace of buffer C alone is also shown. Bottom panel: The average of 4, 2, and 2 traces are shown for fet3Δ, mrs3Δmrs4Δ, and ccc1Δ cells. These cells were grown in medium supplemented with 100, 40, and 40 μM FeIII citrate (see Table 1). The FTS from the fet3Δ cells were incubated with acid phosphatase (PPX) as described.37.
