Fig. 8. Copper-detected chromatograms of cytosolic FTSs. (A) and (B) From WT cells grown with 40 μM Fe and 50 μM Cu; isolated using buffer (C). Green, purple, and red lines are simulations of peaks at 1000, 600, and 400 Da, respectively. The black line is a composite simulation. (C) and (D) From WT cells grown with 40 μM Fe and 10 μM Cu; isolated using buffer (C). (E) From WT cells grown with 40 μM Fe and 10 μM Cu; isolated using 20 mM ammonium acetate. (F) Buffer only (20 mM ammonium acetate). (A) Single treated column was used.
