Table 1. Catalog of isolated cytosol batches used in this study. Metal concentrations are in μM. MM, minimal medium; CSM, complete synthetic medium.
Strain | FeIII citrate | CuSO4 | ZnCl2 | MnCl2 | Media | Num. of batches |
W303 | 1 | 10 | 0 | 0 | MM | 8 |
W303 | 40 | 10 | 0 | 0 | MM | 4 (plus 2) |
W303 | 100 | 10 | 0 | 0 | MM | 4 |
W303 | 10 000 | 10 | 0 | 0 | MM | 2 |
W303 | 40 | 0 | 0 | 0 | MM | 3 |
W303 | 40 | 50 | 0 | 0 | MM | 2 (plus 2) |
W303 | 40 | 10 | 100 | 0 | MM | 2 |
W303 | 40 | 10 | 100 | 40 | MM | 2 |
fet3Δ | 100 | 10 | 0 | 0 | MM | 4 |
ccc1Δ | 40 | 10 | 0 | 0 | MM | 2 |
mrs3Δ mrs4Δ | 40 | 10 | 0 | 0 | MM | 2 |
vma2Δ | 40 | 10 | 0 | 0 | MM | 2 |
cox17Δ | 40 | 10 | 0 | 0 | MM | 2 |
cup1Δ | 40 | 10 | 0 | 0 | CSM | 2 |
CUP1R | 40 | 10 | 0 | 0 | MM | 2 |