Fig. 10. Growth characteristics of the wild type and two mutant estrogen receptor forms in MCF7 cells grown in 3D MPS and 2D static culture models. A) Image set of mCherry expressing MCF7 mutant Y537S cells collected over 17 days. Image processing in ImageJ used to quantify the clonal growth of the wild type and two estrogen receptor mutant MCF7 cells. Additional images are found in ESI† Fig. S5. B) Results at days 5, 9 13 and 17 collected under microfluidic flow from MCF7 wild type, MCFY mutant D538G and mutant Y537S mutant forms of the estrogen reception. Under microfluidic flow, the MCF7 cell Y537S mutant presents robust clonal expansion compared to the MCF7 wild type and D538G mutant form. C) Results collected in static, monolayer cultures of MCF7 found the wild type, MCF7 mutant D538G and mutant Y537S mutant forms of the estrogen reception. When cultured as 2D static culture, the D538G and Y537S mutant MCF7 cells have a slight growth advantage compared to the MCF7 wild type. Green = wt, blue = D538G, red = Y537S. Fig. S16† shows an example of the metadata associated with the images.