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. 2020 May 6;22(5):1244–1254. doi: 10.1007/s11307-020-01499-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2. — Long-term fate of hESCs and DAPCs. a Schematic of injection and experimental timeline of hESC administration and imaging. b Representative RARE MRI scan (day 27) and corresponding histological section (H&E staining) of a rat that received undifferentiated hESCs (left hemisphere: non-transduced, right hemisphere: Fluc-ZsGreen⁺). Both sides display a large area of hyperintense contrast at the injection site (arrowheads) which was confirmed to correspond to tightly packed cell nuclei via histology. c Fluorescence microscopy of areas of abnormal growth in the right hemisphere. In all cases, the growth corresponded to cells of human origin, as evidenced by expression of a human nuclear antigen. The level of ZsGreen expression was heterogeneous within the growths, with areas of strong expression (top) and areas where ZsGreen was lost (bottom). Scale bar = 50 μm. d Schematic of injection and experimental timeline of DAPC administration and imaging. e BLI of two of the six rats that received DAPCs as imaged on days 1, 14 and 91. Most, but not all, rats displayed a signal on the injection day, but this was lost by day 14, and no signal was seen at any other time points. The left panel is representative of rats that displayed signal on day 1, and the right panel representative of rats that did not display a signal on any of the days. Data for the other rats and time points are shown in the ESM. Note that this rat strain can display cycles of thin hair growth, as seen in some images. f RARE MRI scans (day 90) of all six rats that received DAPCs (left hemisphere: non-transduced, right hemisphere: Fluc-ZsGreen⁺). No abnormal features are seen, apart from the needle track that is still visible in some animals (indicated arrowheads in the first rat only).