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. 2020 Sep 3;11:1010. doi: 10.3389/fphys.2020.01010

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Copyright © 2020 Liu, Di, Hu, Zhao, Xu, Wang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structural changes of lacrimal glands in AQP5^–/– mice compared to AQP5^+/+ mice. (A) Hematoxylin–eosin staining of lacrimal glands from AQP5^+/+ mice and AQP5^–/– mice. Arrows indicate vacuoles inside the cytoplasm of AQP5^–/– acinar cells. (B) The number (I) and the area of single acini (II) in the unit area, the number of lacrimal gland epithelial cells in the unit area (III), and the number (IV) and area of vacuoles (V) in the unit area were compared between AQP5^–/– and AQP5^+/+mice. The data are expressed as mean ± SD (n = 6 per group). *p < 0.05. (C) Transmission electron microscopy of lacrimal glands from AQP5^+/+ mice and AQP5^–/– mice. N, nuclei; Nu, nucleolus; M, mitochondrion; RER, rough endoplasmic reticulum; SG, secretory granule; TJ, tight junction; DE, desmosome; AP, autophagosome; triangles, intercellular space.