Table 3.

FACS analysis of tumour-infiltrating leukocyte (TIL) populations from MC38 tumour-bearing mice at day 14 post-induction of ICI monotherapy or combination therapies. Percentages of T cell subpopulations across control groups, treatment responder (TR) arms, and all treatment non-responders (TNR) across all treatment arms. Data are shown as mean % of cells ± SD and are representative of n = 5–10 mice/ group. *P < 0.05; **P < 0.01, comparing TR to TNR

	MC38
	CD3+ % of CD45+	CD8+ % of CD3+	CD8+ GZB+ % of GZB+
Control	12.2 ± 3.0	64.5 ± 1.9	33.9 ± 4.1
TR
αPD1	17.8 ± 5.7	74.7 ± 5.6*	48.0 ± 4.6*
αCTLA4	16.4 ± 5.9	76.2 ± 6.5*	49.2 ± 6.3*
αPD1 + αCTLA4	24.7 ± 10.1	78.1 ± 4.9*	53.6 ± 2.3*
αOX40	29.5 ± 11.3*	74.2 ± 6.3*	54.4 ± 3.5*
αPD1 + αOX40	32.3 ± 4.8*	78.0 ± 6.7*	64.8 ± 4.7**
αTIM3	13.8 ± 3.1	72.8 ± 1.8*	51.2 ± 6.9*
αPD1 + αTIM3	16.3 ± 4.1	73.4 ± 4.5*	49.2 ± 11.7*
αLAG3	17.0 ± 3.6	74.6 ± 2.4*	47.9 ± 5.1*
αPD1 + αLAG3	18.1 ± 4.4	76.5 ± 5.2*	43.5 ± 4.6*
TNR	12.8 ± 2.8	49.9 ± 1.8	32.4 ± 9.6