Table 2.
Validation data obtained with the DSI GC-MS/MS method in liver extracts
| Analyte | Abbreviation | LOD (pg μL−1) | LOQ (pg μL−1) | Linearity (R2) | ME (%) | Intraday precision RSD (%) (n = 10) | Mean relative intraday recovery (%) (n = 10) | Interday precision RSD (%) (n = 12) | Mean relative interday recovery (%) (n = 12) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 20 pg μL−1 | 500 pg μL−1 | 20 pg μL−1 | 500 pg μL−1 | 20 pg μL−1 | 500 pg μL−1 | 20 pg μL−1 | 500 pg μL−1 | ||||||
| Tributyl phosphate | TBP | 35.2 | 106.6 | 0.994 | 103 | n.d. | 5.0 | n.d. | 89 | n.d. | 10.3 | n.d. | 86 |
| Atrazine | ATZ | 1.4 | 4.2 | 0.999 | 102 | 3.3 | 2.9 | 94 | 98 | 4.6 | 1.3 | 93 | 101 |
| Tris(2-chloroethyl) phosphate | TCEP | 7.2 | 21.8 | 0.999 | 105 | n.d. | 3.4 | n.d. | 105 | n.d. | 3.6 | 96 | 96 |
| Diazinon | DAZ | 1.8 | 5.3 | 0.999 | 105 | 2.2 | 0.7 | 103 | 94 | 1.0 | 0.7 | 103 | 94 |
| 2,4,4′-Trichlorobiphenyl | PCB 28 | 3.7 | 11.4 | 0.999 | 100 | 3.1 | 0.4 | 103 | 99 | 1.9 | 0.7 | 102 | 99 |
| Chlorpyrifos-methyl | CPM | 1.8 | 5.6 | 0.999 | 99 | 1.6 | 1.0 | 109 | 100 | 1.0 | 0.6 | 108 | 100 |
| 2,2′,5,5′-Tetrachlorobiphenyl | PCB 52 | 3.4 | 10.3 | 0.999 | 100 | 2.5 | 0.5 | 105 | 98 | 5.3 | 0.5 | 105 | 98 |
| Metolachlor | MTC | 2.0 | 6.0 | 0.999 | 101 | 1.0 | 0.3 | 102 | 97 | 0.9 | 0.8 | 103 | 97 |
| Chlorpyrifos-ethyl | CPE | 2.3 | 6.9 | 0.999 | 100 | 1.3 | 1.0 | 97 | 98 | 1.3 | 0.8 | 96 | 97 |
| Bromophos-methyl | BOM | 1.8 | 5.5 | 0.999 | 113 | n.d. | 4.3 | n.d. | 94 | n.d. | 2.2 | n.d. | 88 |
| Irgarol | IGL | 2.1 | 6.2 | 0.993 | 100 | n.d. | 7.5 | n.d. | 97 | n.d. | 6.8 | n.d. | 100 |
| Fipronil | FPL | 7.1 | 21.7 | 0.999 | 166 | 12.0 | 7.2 | 105 | 145 | 12.4 | 9.2 | 126 | 116 |
| Bromophos-ethyl | BOE | 1.5 | 4.4 | 0.999 | 119 | 3.2 | 2.9 | 95 | 94 | 3.6 | 1.6 | 86 | 87 |
| 2,2′,4,5,5′-Pentachlorobiphenyl | PCB 101 | 2.4 | 7.3 | 0.999 | 100 | 3.5 | 0.5 | 93 | 97 | 2.1 | 0.9 | 91 | 97 |
| p,p’-Dichlorodiphenyldichloroethylene | DDE | 2.7 | 8.1 | 0.999 | 74 | 10.5 | 7.9 | 111 | 91 | 11.5 | 7.0 | 113 | 99 |
| Flamprop-methyl | FPM | 4.4 | 13.3 | 0.999 | 146 | n.d. | 5.4 | n.d. | 103 | n.d. | 5.7 | n.d. | 91 |
| Chlorfenapyr | CFP | 29.1 | 88.2 | 0.998 | 135 | n.d. | 6.8 | n.d. | 107 | n.d. | 12.2 | n.d. | 85 |
| 2,3′,4,4′,5-Pentachlorobiphenyl | PCB 118 | 3.1 | 9.4 | 0.999 | 100 | 2.1 | 0.7 | 95 | 99 | 1.5 | 0.5 | 94 | 99 |
| p,p’-Dichlorodiphenyldichloroethane | DDD | 2.5 | 7.7 | 0.999 | 103 | 8.5 | 4.0 | 98 | 103 | 18.0 | 9.9 | 95 | 94 |
| 2,2′,4,4′,5,5′-Hexachlorobiphenyl | PCB 153 | 1.8 | 5.3 | 0.999 | 100 | 2.3 | 0.5 | 98 | 98 | 1.9 | 0.6 | 97 | 98 |
| p,p’-Dichlorodiphenyltrichloroethane | DDT | 1.0 | 3.0 | 0.999 | 97 | 2.0 | 1.2 | 95 | 99 | 9.6 | 1.2 | 96 | 97 |
| 2,2′,3,4,4′,5′-Hexachlorobiphenyl | PCB 138 | 3.3 | 10.1 | 0.999 | 102 | 2.8 | 1.0 | 99 | 98 | 1.6 | 0.6 | 99 | 98 |
| Triphenyl phosphate | TPP | 26.5 | 80.4 | 0.997 | 101 | n.d. | 10.3 | n.d. | 147 | n.d. | 9.6 | n.d. | 108 |
| p,p’-Dimethoxydiphenyltrichloroethane | MOC | 5.0 | 20.0 | 0.998 | 101 | n.d. | 5.4 | n.d. | 111 | n.d. | 6.7 | n.d. | 115 |
| 2,2′,3,4,4′,5,5′-Heptachlorobiphenyl | PCB 180 | 2.3 | 7.0 | 0.999 | 97 | 2.7 | 1.0 | 100 | 99 | 1.6 | 0.7 | 99 | 99 |
| Tris(2-methylphenyl) phosphate | TMPP | 3.0 | 9.1 | 0.999 | 38 | 7.7 | 6.2 | 122 | 132 | 15.4 | 12.2 | 151 | 137 |
| 2,2′,3,3′,4,4′,5,5’-Octachlorobiphenyl | PCB 194 | 2.5 | 7.6 | 0.999 | 88 | 4.7 | 4.4 | 96 | 110 | 6.9 | 6.5 | 122 | 124 |