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. 2020 Sep 16;8(2):e001052. doi: 10.1136/jitc-2020-001052

Figure 1.

Figure 1

Clinical course. (A) LDH (lactate dehydrogenase) and MRD (genetic minimal residual disease) are displayed as leukemic markers. Red boxes indicate major events in the patient’s history. (B) CD19 expression as measured by flow cytometry (clone SJ25C1, BD) was seen on bone marrow aspirates at initial diagnosis, third relapse and peripheral blood on day +65 after second CD19-CAR T-cell therapy. At the time of invasive ocular diagnostics, 12 days after ocular symptom onset on day +28 after second CD19-CAR T-cell therapy, no CD19 expression was detected within the bone marrow. (C) Flow cytometry analysis of second CD19-CAR product demonstrating a CD4:CD8 ratio of 7.6:1. (D) flow cytometry analysis of CD19-CAR transduction of second CD19-CAR product. FITC-labeled recombinant CD19 protein was used to detect CD19-CAR transduced CD3+ cells within the CAR product. A negative control using non-transduced T-cells is displayed as reference. (E) CD95 expression on CD3+ T cells in the second CD19-CAR product was measured by flow cytometry (red histogram, “second CAR T-cell product”). as reference, non-transduced T-cells were included in the histogram (orange histogram ‘control T cells’). B, blinatumomab cycle; BM, bone marrow; CAR, chimeric antigen receptor; CNS, central nervous system; CTx, chemotherapy; HSCT, hematopoietic stem cell transplantation; I, inotuzumab ozogamicin cycle; P, pembrolizumab cycle; RT, radiotherapy; SSC-A: side scatter area; FITC: Fluorescein isothiocyanate.