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. 2020 Sep 16;8(2):e000421. doi: 10.1136/jitc-2019-000421

Figure 1.

Figure 1

Cryo-thermal therapy-induced systematic and long-term antitumor immunity. (A) Kaplan-Meier survival curve. Cryo-thermal therapy and RFA improved long-term survival in comparison with untreated group. Approximately 5×105 B16F10 cells were injected subcutaneously into the right flank of each mouse. Twelve days later, mice were treated with RFA or cryo-thermal therapy, and untreated mice were as control. Kaplan-Meier survival curve was compared using log-rank tests. ***P<0.001, ****p<0.0001. n=12 for each group. (B) Distant tumor growth of mice treated with cryo-thermal therapy or RFA. Seven days after 5×105 B16F10 cells implanted subcutaneously into the right flank, 1×105 B16F10 cells were injected subcutaneously into the left flank. Another 5 days later, mice were treated with RFA or cryo-thermal therapy, and untreated mice were set as control. Kaplan-Meier survival curve was compared using log-rank tests. *P<0.05, **p<0.01. n=4 for each group. (C) B16F10 rechallenge on mice treated with cryo-thermal therapy or RFA. Upper: schematic of experimental design. Mid: photographic images of lungs from cryo-thermal or RFA-treated and untreated mice, respectively. Lower: quantitative statistics of tumor nodules in lung. All data were shown as mean±SD. n=6 per group. **P< 0.01, ****p<0.0001. Data for graphs were calculated using one-way ANOVA. ANOVA, analysis of variance; RFA, radiofrequency ablation.