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. 2020 Sep 16;8(2):e000421. doi: 10.1136/jitc-2019-000421

Figure 3.

Figure 3

Adoptive T-cells therapy using expanded T-cells from mice treated with cryo-thermal therapy and RFA. (A) Schematic of experimental design. (B, C) Frequency of IFN-γ+ cells in CD8+ T-cells and CD4+ T-cells stimulated with αCD3 and then expanded with IL-2. Mean percentages of IFN-γ+ CD8+ T-cells (B) and IFN-γ+ CD4+ T-cells (C) in each group were shown (data for bar graphs were calculated using two-way ANOVA, *p<0.05), n=4 for each group. (D) Tumor nodules in lung after adoptive T-cells therapy. Left: photographic images of lungs from mice receiving expanded T-cells from naïve mice, untreated, cryo-thermal-treated and RFA-treated mice, respectively. Right: quantitative statistics of tumor nodules in lung. All data were shown as mean±SD n=6 per group. ***P< 0.001, ****p<0.0001. Data for graphs were calculated using one-way ANOVA. ANOVA, analysis of variance; IFN-γ, interferon-γ; RFA, radiofrequency ablation.