Table 1.
The primer sets used for RT-PCR and real-time qPCR analysis.
| Gene | Genus | Forward primer | Reverse primer |
|---|---|---|---|
| NSP12 | PEDV | GGCTTATTTAAACGAGTACGGGGCT | TTGTAAAACTGCAGATTTCTCATA |
| IFN-α | Porcine | CAGTTCTGCACTGGACTGGA | CACAGGGGCTGTAGCTCTTC |
| Rat | CTGTCCTGCATGAGCTGACC | GGAGATTCCTGCACCCCTAC | |
| Chlorocebus sabaeus | CAGAGACTCCCCTGATGAACAA | CCAGGCACAAAGGCTGTATTTC | |
| IFN-β | Porcine | TGCAACCACCACAATTCC | CTGAGAATGCCGAAGATCTG |
| Rat | ACTTGGGTGACATCCACGAC | AAGACTTCTGCTCGGACCAC | |
| Chlorocebus sabaeus | GAGCCGTAGTGGAGAAGCAC | GGCCATACCCATCTAACTGC | |
| IFN-γ | Porcine | GCCATTCAAAGGAGCATGGAT | CTGATGGCTTTGCGCTGGAT |
| Rat | CATCGCCAAGTTCGAGGTGA | TCTGGTGACAGCTGGTGAATC | |
| Chlorocebus sabaeus | TGAATGTCCAACGCAAAGCA | TCGACCTCGAAACATCTGACT | |
| IFN-λ1 | Porcine | TGGCCTTAGAGGCTGAGCTA | CCCTGATGCAAGCCTGAAGT |
| Rat | GGCCAAGGATGCCATTGAAG | GGGTCAGTCATGTTCCCCAA | |
| Chlorocebus sabaeus | AATCTCTGTCACCGCAAGAGT | TCACCTGGAGAAGCCTTAGGT | |
| IFN-λ3 | Porcine | GTCCCTCTTGGAGGACTGGA | TGCTGTGCAGGGATGAGTTC |
| Rat | GGCCAAGGATGCCATTGAAG | TGGGTCAGTCATGTTCCCCA | |
| IL-1β | Porcine | GGCCATAGTACCTGAACCCG | CCAAGGTCCAGGTTTTGGGT |
| Rat | GATGAGGACCCAAGCACCTT | GTCGTCATCATCCCACGAGT | |
| Chlorocebus sabaeus | GTGGCAACGAGGATGACTTG | GCCCTTGTTGTAGTGCTCGT | |
| IL-6 | Porcine | AATGCTCTTCACCTCTCC | TCACACTTCTCATACTTCTCA |
| Rat | AGAGACTTCCAGCCAGTTGC | AGTCTCCTCTCCGGACTTGT | |
| Chlorocebus sabaeus | TTCGGTCCAGTTGCCTTCTC | CTGAAGAGGTCAGTGGCTGG | |
| IL-8 | Porcine | CTGAGAGTGATTGAGAGT | TTACTGCTGTTGTTGTTG |
| Rat | CTCCAAACCTTTCCACCCCA | TTCCTTGGGGTCCAGACAGA | |
| Chlorocebus sabaeus | GTGGACCACACTGCGTCAAT | CTCCACAACCCTAGACACCC | |
| IL-17A | Porcine | AAGTCCAGGATGCCCAAA | CGGTTCAAGATGTTCAAGTTG |
| Rat | CTGAGGGGAAGTTGGACCAC | TAGAGGAAACGCAGGTGCAG | |
| Chlorocebus sabaeus | AACCGGAATACCAATACCAGTCTC | CTCTCAGGGTCCTCATTGCG | |
| TNF−α | Porcine | GTCTCAAACCTCAGATAAG | GTTGTCTTTCAGCTTCAC |
| Rat | TCGTAGCAAACCACCAAGCA | GTGAGGAGCACATAGTCGGG | |
| Chlorocebus sabaeus | CCTGGTATGAGCCCATCTACCTA | AGTCGGGCAGATTGATCTCAG | |
| GAPDH | Porcine | CAAGAAGGTGGTGAAGCAGG | ACCAGGAAATGAGCTTGACG |
| Rat | GCCATCAACGACCCCTTCAT | AGATGGTGATGGGTTTCCCG | |
| Chlorocebus sabaeus | AAATCAAGTGGGGCGATGCT | ACAAACATAGGGGCGTCAGC |