REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Laminin	Abcam	ab11575, RRID:AB_298179
Anti-Paxillin	Abcam	ab32084, RRID:AB_779033
Anti-E-cadherin	BD	610181, RRID:AB_39758
Anti-Ezrin	BD	610602, RRID:AB_397939
Anti-Occludin	Thermo Fisher	71-1500, RRID:AB_2533977
AlexaFluor 568 donkey anti-mouse	Invitrogen	A-11031, RRID:AB_144696,
AlexaFluor 568 donkey anti-rabbit	Invitrogen	A-11036, RRID:AB_143011
Chemicals, Peptides, and Recombinant Proteins
Formaldehyde solution	Sigma-Aldrich	F8775
MEM, no phenol red	Thermo Fisher	Cat#51200087
Glycine	Sigma-Aldrich	G8898
Gelatin 40-50% in H2O	Sigma-Aldrich	G7765
EDTA	Sigma-Aldrich	EDS
Saponin	Sigma-Aldrich	47036
PBS	Gibco	Cat#18912014
Phalloidin488	Thermo Fisher	A12379
Hoechst 33342	Invitrogen	H3570
DMEM	Gibco	Cat#0566016
Penicillin-Streptomycin	Gibco	Cat#15070063
Nonessential amino acids (NEAA) 100X	Gibco	Cat#11140050
FBS	Gibco	Cat#10270106
Alginate lyase	Sigma-Aldrich	A1603
Fluorescein (FITC)	Sigma-Aldrich	46955
Blebbistatin	Sigma-Aldrich	B0560
Mitomyocin C	Sigma-Aldrich	M4287
L-15 Medium (Leibovitz)	Sigma-Aldrich	L1518
Matrigel Matrix	Corning	354230
Low-melting agarose	Sigma-Aldrich	A9414
Alginate	FMS BioPolymer	Protanal LF200FTS
ATTO647N-amine	ATTO-TEC	AD647N-95
Fluoresceinamine	Sigma-Aldrich	201626
DMSO (anhydrous)	Invitrogen	Cat#D12345
Sulfo-NHS	Sigma-Aldrich	56485
EDC	Sigma-Aldrich	03449
MES	Sigma-Aldrich	3671
SDS 20% solution	Sigma-Aldrich	428018
Critical Commercial Assays
pLenti6.3/V5-DEST™Gateway™ Vector Kit	Invitrogen	Cat#V53306
Deposited Data
All Imaging raw data	Roux Lab	Trushko -> Data
Raw data and custom analysis codes	Mendeley Data	https://doi.org/10.17632/3vfxhr2m34.1
Experimental Models: Cell Lines
Madin-Darby Canin Kidney II (MDCK-II)	ECACC	Cat#00062107
MDCKII Myr-PALM-GFP	A gift from the lab of Dr. Mathieu Piel (Institut Curie, Paris, France)	N/A
MDCKII H2B-eGFP mCherry-Actin	A gift from the lab of Prof. Daniel J. Müller (BSSE, ETH Zurich, Switzerland)	N/A
MDCKII Myr-PALM-GFP H2B-mCherry	This paper	N/A
Recombinant DNA
H2B-mCherry	A gift from Robert Benezra (Memorial Sloan Kettering Cancer Center, Sloan Ketting Institute, New York, US)	Addgene plasmid #20972, RRID:Addgene_20972
pLenti6.3/V5-DEST containing C-terminal mCherry	This paper	N/A
Software and Algorithms
MATLAB	MATLAB	N/A
Fiji	https://fiji.sc/	N/A
ImageJ	ImageJ	N/A
SolidWorks	Dassault Systèmes	N/A
Illustrator CS6	Adobe	N/A
Origin	OriginLab Corp.	N/A
Imaris	Oxford Instrument	N/A
Other
Micro Hi-Res Plus 3D Printer	EnvisionTEC	N/A
Resin for 3D printer	EnvisionTEC	HTM 140 V2
PFA Tubing, 360 μm OD, 100 μm ID	IDEX	1932
Loctite EA M-31 CL	Henkel	N/A
Dremler 8000	Dremler	F0138000JF
Dremler Workstation (220)	Dremler	26150220AA
Pumps neMESYS (low pressure module V2)	Cetoni	N/A
Glass Syringes 10mL	Analytical Science	10MDR-LL-GT SGE
Bohlender PTFE Tubings 0.5 mm ID	Aldrich	Z609706
Copper ring, 21 mm OD	RadioSpare (RS)	Cat#367-6177
High Voltage DC Power Supply	Stanford Research	PS350
Glass-bottom dish	MatTek	P35G-1.0-14-C
Slide-A-Lyzer™ Dialysis cassette 10K 12-30mL capacity	Thermo Fisher	Cat#66456
Acrodisc Syringe Filter with Glass Fiber – 1um	Pall	4529
Micro-mechanical testing machine	FemtoTools	FT-FS1000
Microforce sensing probe	FemtoTools	FT-S100
Microscope LSM780	Carl Zeiss	N/A
Objective Plan-Apochromat 20x/0.8 M27	Carl Zeiss	420650-9902-000
Microscope LSM710	Carl Zeiss	N/A
Objective W Plan-Apochromat 20x×/1.0 DIC M27 75mm	Carl Zeiss	421452-9800-000