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. 2020 Feb 14;124(10):1941–1948. doi: 10.1021/acs.jpcb.0c00770

Table 1. Equilibrium Constants K Determined by FRET and BRIGHTNESS method for Double Labeled Pairs of Oligonucleotides.

  K × 109 M–1
    brightness method
  FRET blue channel red channel
3′488/647N 3.5 ± 1.9 2.9 ± 0.9 3.9 ± 1.1
488/647N 1.1 ± 0.5 0.9 ± 0.7 1.4 ± 0.9