Fig. 7.

The ability of obtained phosphoroorganic peptide mimetics to induce autoubiquitination and proteasomal degradation of cIAP1. Western blot analysis of MDA-MB-231 cell lysates subjected to incubation with reference compounds: G – GDC-0152, L – LCL-161, selected phosphoroorganic peptide derivatives (13, 14, 41 and 42) and C – negative control treated only with DMSO; A and B – samples taken after 15 and 120 min incubation with antagonists (a). The dose response for compound 41 (1, 2.5, 5 and 10 µM) on the degradation of cIAP1 protein after 15 min incubation with MDA-MB-231 cells; C – negative control treated only with DMSO (b)