. Author manuscript; available in PMC: 2020 Dec 17.

Published in final edited form as: Anal Chem. 2019 Nov 22;91(24):15709–15717. doi: 10.1021/acs.analchem.9b03879

Table 1.

Identified inter cross-links with BS³, BS²G and EDC cross-linking.

	IL-7	IL-7Rα	Cross-linker	Reported Cross-linkable Cα- Cα Distance, Å	Measured Cα- Cα Distance in the Generated Model, Å
1	K11	K84	BS³	9-30⁵²	14.3
2	K11	K141	BS³	9-30⁵²	13.9
3	D75	K77	EDC	6-16⁵³	9.8
4	K69	K78	BS³	9-30⁵²	17.2
5	K152	K141	BS³	9-30⁵²	24.2
6	K8	K84	BS²G / BS³	6-26/9-30⁵²	12.3^a
7	N-term	K84	BS²G / BS³	6-26/9-30⁵²	-
8	D2	K84	EDC	6-16⁵³	-
9	D4	K84	EDC	6-16⁵³	-
10	E6	K84	EDC	6-16⁵³	-

Cross-linked distance is measured at the Cα of D9 instead of K8 in crystal structure (PDB: 3DI2) because the N-terminal region from M1-K8 is missing in the X-ray structure.