Figure 1.
PCP Is Normal with Membrane-Tethered Wingless and the Absence of DWnt4
(A) A model of how a Wnt gradient may lead to a Fz “activity” gradient that directs the asymmetric localization of core PCP protein complexes, eventually polarizing hair outgrowth in one direction. Note that PCP is initially radial toward the prospective wing margin, where Wg and Wnt4 are expressed. The PCP axis subsequently reorient to the final proximal-distal pattern during morphogenesis (Aigouy et al., 2010). For simplicity, the Wnt gradient and PCP are shown aligned with the proximal-distal axis throughout.
(B) A wild-type adult wing (B′) and a 28 h APF pupal wing with anti-Stan staining (B″). Here and in subsequent figures, the orientation and magnitude of Stan's asymmetric localization in each cell is depicted by magenta lines. Stan orientation data from the pupal wing region (corresponding to the region marked by a red rectangle in the adult wing) was tabulated on a polar coordinate histogram, with n denoting the number of cells (pooled from 4 pupal wings).
(C and D) Wing, pupal wing, and Stan orientation polar histogram for homozygous NRT-Wg larvae (no diffusible Wingless) (C) or for homozygous NRT-Wg larvae lacking DWnt4 (D). Conversion to NRT-Wg was triggered by GAL4-driven Flp, which has been shown to be efficient previously (Hadjieconomou et al., 2011; Bosch et al., 2017). The p values were calculated with a two-sample Kolmogorov-Smirnov test that compares the distribution of the Stan orientation in mutant conditions (C″ and D″) with that in the wild type (shown in B″).
(E) Wing from a hemizygous fz1P21 mutant fly.
(E') Pupal wing and polar histogram of Stan orientations for the same genotype. Scale bars are 50 μm unless specified otherwise.