REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
mouse anti-Stan	DSHB	Cat# Flamingo #74 RRID:AB_528247
rat anti-shg	DSHB	Cat# DCAD2 RRID:AB_528120
mouse anti-Wg	DSHB	Cat# 4D4 RRID: AB_528512
rabbit anti-GFP	Abcam	Cat# ab6556 RRID:AB_305564
Alexa Fluor 488 Goat anti-Rat IgG (H+L) Cross-adsorbed Secondary Antibody	Thermo Fisher Scientific	Cat# A-11006 RRID:AB_2534074
Alexa Fluor 488 Goat anti-Mouse IgG (H+L)	Thermo Fisher Scientific	Cat# A28175 RRID:AB_2536161
Alexa Fluor 555 Plus Goat anti-Mouse IgG (H+L) Highly Cross-adsorbed Secondary Antibody	Thermo Fisher Scientific	Cat# A32727 RRID:AB_2633276
Alexa Fluor 647 Goat anti-Rat IgG (H+L) Cross-adsorbed Secondary Antibody	Thermo Fisher Scientific	Cat# A-21247 RRID:AB_141778
Alexa Fluor 647 Donkey anti-Rabbit IgG H&L	Abcam	Cat# ab150075 RRID:AB_2752244
Chemicals, Peptides, and Recombinant Proteins
16% formaldehyde, methanol free	Thermo Fisher Scientific	Cat# 28906
Vectashield with DAPI	Vector Laboratories	Cat# H-1200 RRID:AB_2336790
Experimental Models: Organisms/Strains
wg[cNRT]	Alexandre et al., 2014	N/A
DWnt4KO, wg[cNRT]	This paper	N/A
rngal4	Bloomington Drosophila Stock Center	RRID:BDSC_7405 Flybase: FBal0137099
UAS-Flp(III)	Bloomington Drosophila Stock Center	RRID:BDSC_4540 Flybase: FBst0004540
gfp-wg	Port et al., 2014	N/A
nlsGFP-DWnt2	This paper	N/A
nlsGFP-DWnt4	This paper	N/A
nlsGFP-DWnt5	This paper	N/A
nlsGFP-DWnt6	This paper	N/A
Dwnt10[HA]	This paper	N/A
Dwnt10[GAL4]	This paper	N/A
DWntD[GAL4]	This paper	N/A
Dwnt2KO, Dwnt4[cKO], wg[cNRT], Dwnt6[KO], Dwnt10[KO]	This paper	N/A
UAS-Flp5	lab of Iris Salecker	N/A
Fz1P21	Bloomington Drosophila Stock Center	RRID:BDSC_41787 Flybase: FBal0004937
vggal4	This paper	N/A
UAS-NanobodyKDEL	This paper	N/A
wls[ExGFP]	This paper	N/A
nubgal4	Bloomington Drosophila Stock Center	RRID:BDSC_25754 Flybase: FBti0016825
UAS-cas9	Port et al., 2020	N/A
wlssgRNA	Port et al., 2020	N/A
wggal4	Alexandre et al., 2014	N/A
rnlexa	This paper	N/A
lexaop-flp	lab of Iris Salecker	N/A
UAS-FRT stop FRT-hid-2A-reaper	lab of Iris Salecker	N/A
dsh[cKO]	This paper	N/A
Oligonucleotides
Table S1	This work	N/A
Software and Algorithms
Fiji	https://fiji.sc/	N/A
GraphPad Prism	GraphPad Software, Inc.	N/A
MATLAB_R2014b	Mathworks	N/A
Tissue Analyzer	Aigouy et al., 2010https://grr.gred-clermont.fr/labmirouse/software/WebPA/	N/A
Polarity measurement MATLAB scripts	Strutt et al., 2016	N/A