Table 2.

List of whole-genome sequences (WGS) of C. difficile that likely possess agr2M. blast search showed that these WGSs have a region with 80.33–80.37 % nucleotide identity compared with agr2R region of R20291 (FN545816.1) and >95% identity compared with agr2M region of M68 (FN668375.1). Determination of ST/clade was performed using the PubMLST sequence query page

Strain	Accession No.	ST	Clade
DSM 29629	CP016104.1	ST39	Clade 4
DSM 29627	CP016102.1	ST37	Clade 4
CD161	CP029154.1	ST37	Clade 4
CDT4	CP029152.1	ST37	Clade 4
M68	FN668375.1	ST37	Clade 4
CF5	FN665652.1	ST86	Clade 4
DSM28669	CP012323.1	ST109	Clade 4
BJ08	CP003939.1	–*	–*
DSM29637	CP016106.1	ST83	Clade 1
CBA7204	CP029566.1	ST203	Clade 1

*WGS of BJ08 did not have adk (one of the seven housekeeper genes used in MLST), but the sequence of the other six housekeeper genes used to determine sequence type were identical to that of ST37.