Fig. 1.

mAb against PDCoV N protein generated. a Titers of anti-PDCoV N protein mAb from Ascites and cell culture supernatants from a monoclonal hybridoma cell line were analyzed by ELISA. Serum from a mock vector immunized mouse acted as a control. The coating concentration of PDCoV N protein was 5 µg/ml. The optical density (OD) values of each point represent the mean value and standard deviation from three determinations (n = 3). b Purified recombinant PDCoV-N protein samples or lysates of LLC-PK1 cells that had been transfected with pCAGGS-HA-PDCoV N or infected with PDCoV were subjected to western blotting with anti-PDCoV N protein mAb. c LLC-PK1 cells were infected with PDCoV, then fixed with paraformaldehyde for an IFA. The green and blue signals indicate PDCoV N and DAPI, respectively. Fluorescent cells were imaged using confocal laser scanning microscopy