Fig 2. RTN3L&S interact with double-stranded viral RNA in HCV infected Huh7 cells.

(A) RNA ChIP analysis was performed from cell lysates from Huh7.0, Huh7.0+JFH-1 infected, and HCV FL-Replicon cells. Immuno-precipitations of cell lysates were performed using specific dsRNA and non-specific IgG antibodies using protein A/G pulldown. (A) Pull-down proteins interacting with dsRNA were probed by western blotting targeting for RTN3L&S and HCV NS3. Input total lysates were subjected to western blot analysis and probed for β-Actin. (B) Total RNA was isolated from Immuno-precipitations of cell lysates following the pulldown of RTN3L&S and non-specific IgG antibodies. Total RNA extracted was subjected to RT-qPCR targeting (B) negative-sense HCV RNA and (C) miR-122. miR-Cel39 RNA served as an exogenous loading control. Data is representative of 3 independently repeat experiments with p<0.05 was considered significant by the Mann–Whitney U test.