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. 2020 Sep 17;15(9):e0236611. doi: 10.1371/journal.pone.0236611

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© 2020 Fatima et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) Nanoparticles in cytosol, vesicles and the nucleus of hCMEC/D3 cells. NPs with 20nt ssDNA (NP-DNA-20^ss) were compared with dsDNA(NP-DNA-20^ds) and the base nanoparticles (NP-Gal). Three experiments were performed, each individual experiment with three technical repeats. Tukey's multiple comparisons test showed a significant difference for NP-DNA-20^ds compared to NP-Gal (** P = 0.0034) and NP-DNA-20^ss (* P = 0.0133) in vesicles. (B) Transmission electron micrographs showing examples of silver enhanced gold NPs in vesicles (NP-DNA-20^ds, A) or the cytosol (NP-Gal, B) of hCMEC/D3 cells after application to the apical surface.