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. 2020 Sep 4;16(9):e1008825. doi: 10.1371/journal.ppat.1008825

Fig 4. Cross-utilization of templates of viruses belonging to the SFV complex in human cells.

Fig 4

HEK293T cells were co-transfected with combinations of each CMV-P1234 replicase expression plasmid or corresponding CMV-P1234GAA control and (A) HSPolI-FG-CHIKV, (B) HSPolI-FG-ONNV, (C) HSPolI-FG-RRV, (D) HSPolI-FG-SFV or (E) HSPolI-FG-MAYV. Transfected cells were incubated at 37°C and lysed 18 h p.t.; cells transfected with plasmids originating from EILV were incubated at 28°C and lysed 48 h p.t. Data represent the luciferase activity (Fluc and Gluc) from CMV-P1234 transfected cells normalized to the paired CMV-P1234GAA control cells. Value obtained for P1234GAA controls was taken as 1; activities lower than that observed for P1234GAA are also shown as 1. X-axis represents different replicases, means + SD are from three independent experiments.