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. 2020 Sep 17;15(9):e0239019. doi: 10.1371/journal.pone.0239019

Fig 2. Melasolv induces autophagy activation in B16F1 cells.

Fig 2

(A, B) B16F1/GFP-LC3 cells were treated with either Melasolv (5–10 μg/ml) or ARP101 (10 μM). After 24 h treatment, the cells were fixed for fluorescence imaging (A). The scale bar indicates 10 μm. The cells with autophagy activation were determined by counting punctate GFP-LC3 dots under a fluorescence microscope (B). Data were obtained from about 200 cells per group and experiments were repeated at least three times. (* p<0.05, SEM, n = 3). (C) B16F1 cells were treated with Melasolv (10 μg/ml) in the presence or absence of bafilomycin A1 (5 nM) for 24 h. The level of LC3 protein was then assessed by Western blotting.