Table 5. Inhibitory Activities of LEI-301 for Metabolic Enzymes of the ECSa.
| remaining enzyme activity at 10 μM LEI-301 (% ± SD; n = 3) | ||||||
|---|---|---|---|---|---|---|
| hNAPE-PLD | hPLA2G4E | mDAGLα | mDAGLβ | hMAGL | mFAAH | mABHD6 |
| 92 ± 8 | 95 ± 5 | 97 ± 10 | 83 ± 1 | 105 ± 19 | 108 ± 4 | 92 ± 5 |
Activities were obtained from surrogate (hNAPE-PLD) or natural (hMAGL) substrate assays. hPLA2G4E, mDAGLα/β, mFAAH, and mABHD6 were determined by gel-based ABPP.