Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Cell line (canine) | MDCK-FUCCI |
Streichan et al., 2014. Wildtype: ECACC-00062107 |
N/A | RFP signal is G1, GFP is G2. |
Other | DMEM - low glucose | Sigma-Aldrich, Inc | Cat.D5523 | |
Other | fetal bovine serum | Atlanta Biologicals | Cat.S11550 | |
Other | Silicone stencil material, 250 µm thick. |
Stockwell Elastomerics | Cat.HT6240-40D | Tissue patterning material |
Software, algorithm | FIJI | NIH ImageJ Project | ||
Software, algorithm | MATLAB | Mathworks, Inc | 2019A | All code compatible back to at least 2015B |
Software, algorithm | Machine Learning Tools | Laboratory code LaChance and Cohen, 2020 | —see References | Full code on GitHub |