Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent | pGEX-KG-GST-Syt1_C2B | Brewer et al., 2015 | Protein expression plasmid for E. coli (rat synaptotagmin-1 C2AB domain, residues 271–421) | |
| Recombinant DNA reagent | pGEX-KG-GST-Syt1_C2AB | Araç et al., 2006 | Protein expression plasmid for E. coli (rat synaptotagmin-1 C2AB domain, residues 140–421) | |
| Recombinant DNA reagent | pMSP1E3D1 | Addgene | 20066 | Protein expression plasmid for E. coli (MSP1E3D1) |
| Chemical compound, drug | DEUTERIUM OXIDE (D, 99.8%) | Cambridge Isotope Laboratories, Inc | DLM-4–99.8 | |
| Chemical compound, drug | AMMONIUM CHLORIDE (15N, 99%) | Cambridge Isotope Laboratories, Inc | NLM-467 | |
| Chemical compound, drug | D-GLUCOSE (1,2,3,4,5,6,6-D7,97–98%) | Cambridge Isotope Laboratories, Inc | DLM-2062 | |
| Chemical compound, drug | L-Methionine-(methyl-13C) | Sigma-aldrich | 299146 | |
| Chemical compound, drug | 2-Ketobutyric acid-4–13C,3,3-d2 sodium salt hydrate | Sigma-aldrich | 589276 | |
| Chemical compound, drug | PC | Avanti polar lipid | 850457 | 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine |
| Chemical compound, drug | PS | Avanti polar lipid | 840035 | 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) |
| Chemical compound, drug | PIP2 | Avanti polar lipid | 840046 | L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine) (ammonium salt) |
| Chemical compound, drug | RhoPE | Avanti polar lipid | 810158 | 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) |
| Chemical compound, drug | Tetramethylrhodamine-5-Maleimide | Invitrogen | T-6027 | |
| Chemical compound, drug | Alexa Fluor488 C5 Maleimide | Thermo Fisher Scientific | A10254 | |
| Chemical compound, drug | β-OG (octyl-beta-glucoside) | Gold biotechnology | O-110–50 | |
| Chemical compound, drug | Detergent Removal Resin | Thermo Fisher Scientific | 87780 |