Table 1.
Translational control of groups of mRNAs during histidine starvation. We used polysomal microarray data to compute the total molar amount of ribosomes bound to a specified group of mRNA in WT and gcn2Δ cells (columns D, E, H, I; odd numbered rows), relative to total amount of ribosomes bound to all 6657 poly(A) RNA species in WT without 3AT. Likewise, we used conventional microarray data from total RNA to compute the total amount of the specified group of mRNAs (columns D, E, H, I; even numbered rows). 3AT Up, the percentage of the value in 3AT compared to that in control. ΔUp, (3AT Up Ribo/3AT Up mRNA) – 1.
| A | B | C | D | E | F | G | H | I | J | K | row # |
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | gcn2- | ||||||||||
| N | Control | 3AT | 3AT Up | ΔUp | Control | 3AT | 3AT Up | ΔUp | |||
| Total poly(A) RNA | 6657 | Ribo # | 100.00% | 81.69% | 81.69% | 1.58% | 85.76% | 83.23% | 97.05% | −1.22% | 1 |
| mRNA | 100.00% | 80.42% | 80.42% | 90.26% | 88.68% | 98.25% | 2 | ||||
| Total mRNA | 5154 | Ribo # | 99.67% | 80.49% | 80.76% | 1.69% | 85.49% | 82.82% | 96.88% | −1.25% | 3 |
| mRNA | 99.36% | 78.90% | 79.41% | 89.63% | 87.94% | 98.11% | 4 | ||||
| Ribosome | 142 | Ribo # | 38.73% | 26.00% | 67.12% | −1.09% | 29.29% | 27.68% | 94.50% | −2.44% | 5 |
| mRNA | 47.01% | 31.90% | 67.86% | 39.66% | 38.42% | 96.87% | 6 | ||||
| Chromatin | 418 | Ribo # | 2.11% | 2.16% | 102.47% | 3.46% | 2.02% | 2.10% | 103.72% | −0.38% | 7 |
| mRNA | 2.02% | 2.00% | 99.05% | 1.98% | 2.06% | 104.12% | 8 | ||||
| Chromatin in TA1 | 262 | Ribo # | 1.01% | 0.98% | 97.01% | 8.01% | 0.96% | 0.94% | 97.28% | −0.22% | 9 |
| mRNA | 1.03% | 0.92% | 89.82% | 0.98% | 0.95% | 97.49% | 10 | ||||
| Chromosome organization | 442 | Ribo # | 2.57% | 2.15% | 83.51% | 0.29% | 2.44% | 2.86% | 117.36% | 2.25% | 11 |
| mRNA | 2.35% | 1.96% | 83.27% | 2.31% | 2.65% | 114.78% | 12 | ||||
| Chromosome organization in TA1 | 264 | Ribo # | 1.02% | 1.00% | 97.62% | 7.98% | 0.96% | 0.95% | 99.30% | 0.26% | 13 |
| mRNA | 1.03% | 0.93% | 90.40% | 0.98% | 0.97% | 99.04% | 14 | ||||
| RNA metabolism | 744 | Ribo # | 10.13% | 7.76% | 76.59% | 4.01% | 8.15% | 8.39% | 102.90% | 0.71% | 15 |
| mRNA | 10.71% | 7.89% | 73.64% | 9.22% | 9.42% | 102.18% | 16 | ||||
| RNA processing | 508 | Ribo # | 8.09% | 5.98% | 74.00% | 4.05% | 6.30% | 6.63% | 105.19% | 1.52% | 17 |
| mRNA | 8.84% | 6.28% | 71.12% | 7.50% | 7.77% | 103.61% | 18 | ||||