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. 2020 Aug 5;48(16):9372–9386. doi: 10.1093/nar/gkaa644

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Free GapR is a tetramer. (A) Elution profiles from size-exclusion chromatography (SEC) for full-length GapR and its truncation mutants GapR^ΔC17, GapR^ΔC8, GapR ^ΔN10. (B) AUC sedimentation analysis of GapR^ΔC17 (concentration 4.0 mg/ml) and GapR (concentrations 1.0, 2.1 and 3.3 mg/ml). The calculated molecular weight values are indicated. (C) SEC-MALS analysis of GapR (concentration 1.0 mg/ml), GapR^ΔC17 (concentration 2.0 mg/ml) and GapR/10A complex (concentration 2.0 mg/ml). Curves for molar mass (g/mol) vs. elution time (min) are shown, with calculated molecular weight values indicated. (D) SEC-MALS analysis of GapR at different concentrations (concentrations 0.5, 1.0 and 2.0 mg/ml).