Table 2.

Equilibrium dissociation constants and thermodynamic parameters for GapR to bind different duplex DNA sequences.

	Sequence	K d(nM)	ΔH (kJ/mol)	ΔTS (kJ/mol)	ΔG (kJ/mol)
10A	F: 5′-CCGAAAAAAAAAACGC	11.6 ± 2.5	46.2 ± 0.4	88.4	–42.2
	R: 5′-GCGTTTTTTTTTTCGG
11AT	F: 5′-TTAAAATTAAA	12.3 ± 4.5	32.2 ± 0.5	74.3	–42.0
	R: 5′-TTTAATTTTAA
5ApT	F: 5′-CCGATATATATATCGC	12.3 ± 8.9	11.1 ± 0.2	53.2	–42.0
	R: 5′-GCGATATATATATCGG
5ApT+NT	F: 5′-CCGATATATATATCGC	16.6 ± 4.5	24.5 ± 0.2	65.8	–41.4
	R: 5′-GCGATATATATATCGG
seq1	F: 5′-GCGAAATTGATCG	72 ± 24	30.6 ± 0.5	68.6	–38.0
	R: 5′-CGATCAATTTCGC
seq2	F: 5′-CGTGTTTTCGG	72 ± 19	30.2 ± 0.4	68.2	–38.0
	R: 5′-CCGAAAACACG
6CpG	F: 5′-CGCGCGCGCGCG	118 ± 17	35.9 ± 0.4	72.8	–36.8
	R: 5′-CGCGCGCGCGCG

The combined standard deviations were calculated from two measurements. (NT: netropsin)