Table 3.
hnRNPK protein domain analysis with the B motif RNA. Average apparent binding constants and associated standard errors observed for the full-length protein and protein mutants with the B motif RNA are listed; n = number of replicates done for each experiment; relative KDs are to that of full-length hnRNPK. Representative binding curves can be found in Supplemental Figures S7B and S20.
| Protein | K D A pp, nM | n | K rel |
|---|---|---|---|
| Full-length | 61 ± 6 | 40 | 1.0 |
| KH1 | DNB | 3 | >160 |
| KH2 | >10 000 | 3 | >160 |
| KH3 | >10 000 | 6 | >160 |
| KH1KH2 | >10,000 | 5 | >160 |
| ΔKH3 | 140 ± 30 | 17 | 2.3 |
| R9 to S9 mutant | 430 ± 40 | 6 | 7.0 |