Figure 1.

Insertion of O395 protospacers in pVCR94 reduces its transfer to Cas⁺V. cholerae O395. (A) Sequence of the protospacers introduced into pVCR94^Sp. (B) Conjugative transfer of pVCR94^Sp derivatives bearing O395 protospacers from E. coli VB111 to V. cholerae O395R (Cas⁺) or O395RΔcas (Cas⁻). (C) Impact of protospacer insertion site on the transfer of pVCR94^Sp to O395R. Positions of V1 protospacer insertions are indicated by orange arrowheads in Figure 2B. Bars are the mean of three independent experiments. Error bars are the standard error of the mean. Statistical significance: ****P< 0.0001 and ***P< 0.001.