Table 1.

Summary of the scar patterns found in rescued pVCR94^Sp carrying V1 protospacer at diverse positions

Protospacer position	Number of sequenced clonesa	Type of scarsb (distribution)	Conjugation properties	Repeat ID used for repairc	Deletion size (bp)d
5	73/85	IA (43/85)	Transmissible	rptIb-rptIf	23 702
		IB (1/85)	Transmissible	rptIb-rptIf	23 702
		IC (7/85)	Transmissible	rptIb-rptIe	14 076
		ID (1/85)	Transmissible	rptIb-rptIe	14 076
		IE (1/85)	Transmissible	rptIb-rptId	14 003
		IF (4/85)	Transmissible	rptIb-rptIc	10 310
		IG (16/85)	Transmissible	rptIa-rptIc	9268
1	0/85	No scar		NA	NA
7	1/85	III (1/85)	Deficient	rptIIIc-rptIIId	6728
3	2/85	IV (2/85)	Deficient	rptIIIa-rptIIIb	59 432
6	9/85	VA (1/85)	Transmissible	rptIIa-rptIIb	5138
		VB (8/85)	Transmissible	rptIVa-rptIVb	4936
2	0/85	No scar		NA	NA
4	0/85	No scar		NA	NA

^a0 values, no repaired transconjugants was obtained.

^bPartial sequences of characterized scars are available in Supplemental Figure S2.

^cAs depicted in Figure 2B. Sequences of repeat IDs are shown in Table 2. NA, not applicable.

^dExact deletion size from pVCR94^Sp-V1 variants