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. 2020 Jul 21;48(16):8927–8942. doi: 10.1093/nar/gkaa596

Figure 2.

Figure 2.

Experimental changes of MRTF-A expression inversely correlate with myogenic differentiation. (A) C2C12 cells were transfected with HA-tagged MRTF-A or control vector (Ctrl.) 48 h prior to induction of myogenic differentiation. (B) Phase contrast images of cells overexpressing HA-tagged MRTF-A. (C) C2C12 differentiation upon partial depletion of MRTF-A using siRNA. Shown are immunoblots for myosin heavy chain polypeptides, HA-tag, myogenin and tubulin of lysates collected at the times indicated. Quantification of relative fluorimetric intensities of three independent experiments are expressed below by bar charts. Error bars, SD (n = 3); *P< 0.05, **P< 0.01 (Student’s t-test). Size bar, 100 μm.