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. 2020 Jul 21;48(16):8927–8942. doi: 10.1093/nar/gkaa596

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Enrichment of miRNAs by binding to the MRTF-A 3′UTR and by myogenic differentiation. (A) Number of sequenced miRNA fragments in miTRAP eluates of undifferentiated or differentiated cells in comparison to the MS2 control. All miRNAs (upper left) and the selected miRNAs are presented independently. (B) Abundance of miRNA sequences in total lysates of undifferentiated and differentiated C2C12. Shown is the expression change of the candidate miRNA during differentiation of C2C12. CPM; counts per million. Error bars, SD (n = 3); *P< 0.05, **P< 0.01, ***P< 0.001 (Student’s t-test).