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. 2020 Jul 21;48(16):8927–8942. doi: 10.1093/nar/gkaa596

Figure 7.

Figure 7.

Expression of miRNA candidates affects the MRTF-A level in murine and human myoblasts. (A) MRTF-A mRNA expression in undifferentiated C2C12 cells transfected with the indicated miRNA for 48 h. A control miRNA (miCtrl.) and a MRTF-A-specific siRNA (siMRTF-A) were used as a negative and positive control, respectively. (B) MRTF-A protein level upon transient miRNAs transfection in C2C12 cells. (C) MRTF-A protein level upon miRNAs transfection for 72 h in primary human HSMM skeletal muscle myoblasts. Normalized protein quantification is shown below each blot. Error bars, SEM (n = 3); *P< 0.05, **P< 0.01, ***P< 0.001 (Student’s t-test).