Skip to main content
. 2020 Jul 21;48(16):8927–8942. doi: 10.1093/nar/gkaa596

Figure 8.

Figure 8.

miR-1a-3p, miR-24-3p and miR-486-5p regulate MRTF-A via recognition sites on its 3′UTR. (A) Schematic of the 3′UTR of murine MRTF-A with the STOP-codon at position 1 and the polyadenylation signal at 985. Deleted miRNA binding sites are indicated by red boxes. (B–F) COS-7 cells were transiently transfected with pMIR-REPORT luciferase reporters, harboring the 3′UTR of murine MRTF-A or mutants thereof with a deletion of the predicted binding sites. The indicated miRNAs were co-transfected and their effects compared to a control miRNA (miCtrl.). After 48 h luciferase activity was determined and normalized to co-transfected galactosidase activity. (G) Decrease of 3′UTR-controlled luciferase activity upon differentiation of C2C12. Cells were transiently transfected with the indicated reporters 48 h prior to myogenic differentiation. Shown is the reporter activity over the course of differentiation, normalized to the vector control (pMIR empty). (H) Relative 3′UTR luciferase reporter activity of the deletion constructs in C2C12 cells following myogenic differentiation for four days. Shown in comparison to MRTF-A wild type 3′UTR reporter which is set as 1. Error bars, SD (n = 3); *P< 0.05, **P< 0.01, ***P< 0.001 (Student’s t-test).