Table 2.
Synergistic interaction of pol2-P301R and Polδ proofreading deficiency
| CAN1 mutation | his7-2 reversion | |||
|---|---|---|---|---|
| Genotype | Mutation rate (×10−7) | Fold increase | Mutation rate (×10−8) | Fold increase |
| POL2/POL2 POL3/POL3 | 3.4 (3.0–4.0) | 1 | 1.1 (0.85–1.3) | 1 |
| POL2/POL2 pol3-D520V/pol3-D520V | 46 (35–69) | 14 | 17 (15–23) | 15 |
| POL2/pol2-P301R POL3/POL3 | 75 (70–93) | 22 | 29 (25–33) | 26 |
| POL2/pol2-P301R pol3-D520V/pol3-D520V | 3100 (2100–4500) | 910 | 2800 (2200–3600) | 2500 |
Mutation rates are medians for at least 18 cultures from two to three independent clones. 95% confidence intervals are shown in parentheses.