Figure 1.

Illustration of sRNA-PAINT probe design and workflow. (A) Illustration of a VARNISH probe design. A VARNISH probe is comprised of three parts: a probe backbone (cyan) that is complementary to the target sRNA (orange), a docking strand (green) and a linker sequence (dark blue) connecting them. The imager strand is conjugated to the fluorophore, and it will find its corresponding docking strand during the imaging process. (B) A workflow for sRNA-PAINT. Samples were fixed and embedded, and a thin section is placed on the coverglass. During the hybridization process, the VARNISH probes will bind to sRNAs in the sample and then locked with EDC. During the imaging step, imager strands are added to the probe-hybridized sample on the coverglass.