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A scaled-up barcoded bead pool allows for the one-pot assembly of up to 1536 genes. (A) Two codon versions of a 1536-gene library were assembled using KAPA HiFi; when combining across both codon usages, 1208/1536 genes have at least one assembly barcode. (B) Comparison of percent perfect assemblies (minimum 100 assembly barcodes) of both codon versions of each 1536-gene library.
