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. 2020 Aug 7;48(16):9262–9272. doi: 10.1093/nar/gkaa652

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Drosophila Oskar eLOTUS exhibits both RNA and protein binding activity. (A) Schematic illustration of secondary structures of Oskar extended LOTUS (eLOTUS) and minimal LOTUS (mLOTUS). (B) Oskar eLOTUS but not mLOTUS interacts with Vasa. GST pull-down assay was performed using GST, GST-Oskar eLOTUS or GST-Oskar mLOTUS against His-Vasa (200–661aa). (C) Both Oskar eLOTUS and mLOTUS interact with RNA G4. ELISA assay was performed with Oskar eLOTUS or mLOTUS and indicated RNA oligos. n = 2. Dissociation constants (K_d) are indicated. (D) A proposed model illustrating different binding modes of mLOTUS and eLOTUS subfamilies.